The Effect of Lithium Periodate on Crystalline Bovine Serum Albumin

نویسندگان

  • Walther F. Goebel
  • Gertrude E. Perlmann
چکیده

A study of the chemical, physicochemical, and immunological changes in bovine serum albumin, brought about by oxidation with lithium periodate, has been made. It has been shown that destruction of certain amino acids occurs, that a change in the absorption spectrum takes place, and that the electrophoretic behavior of the protein is altered. Prolonged contact of bovine albumin with lithium periodate destroys its ability to incite antibodies in experimental animals.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Effect of Different Stabilizers on Stability of Horseradish Peroxidase- Bovine Serum Albumin-Aflatoxin B1, a Conjugated Tracer for Detection of Aflatoxin B1 in Immunoassay-Based Methods

Aflatoxins are a group of fungal toxic metabolites, which are contaminated certain food commodities. ELISA is one of the sensitive methods for detection of aflatoxins. Preparation and stabilizing of a proper conjugated tracer for detection of aflatoxins is probably the main step for designing an ELISA method. In current study, different stabilizers were applied to stabilize a newly prepared con...

متن کامل

The Effect of Different Stabilizers on Stability of Horseradish Peroxidase- Bovine Serum Albumin-Aflatoxin B1, a Conjugated Tracer for Detection of Aflatoxin B1 in Immunoassay-Based Methods

Aflatoxins are a group of fungal toxic metabolites, which are contaminated certain food commodities. ELISA is one of the sensitive methods for detection of aflatoxins. Preparation and stabilizing of a proper conjugated tracer for detection of aflatoxins is probably the main step for designing an ELISA method. In current study, different stabilizers were applied to stabilize a newly prepared con...

متن کامل

Thermodynamic Analysis for Cationic Surfactants Binding to Bovine Serum Albumin

In the present study, the binding isotherms for interaction of a homologous series of n-alkyltrimethyl ammonium bromides with bovine serum albumin (BSA) have been analyzed on basis of intrinsic thermodynamic quantities. In this regards, the intrinsic Gibbs free energy of binding, AGb(i,)„ has been estimated at various surfactant concentrations and its trend of variation for both binding sets ha...

متن کامل

An Inhibitory Effect of Dexamethasone on A549 Cell Adhesion to Neutrophils: Possible Involvement of Nuclear Factor-Kappa B

Abbreviations: AM, acetoxymethylester; BSA, bovine serum albumin; DMEM, Dulbecco’s modified Eagle medium; FITC, fluorescein isothiocyanate; GM-CSF, granulocyte-macrophage colony-stimulating factor; HBSS, Hanks’ balanced salt solution; ICAM-1, intercellular adhesion molecule-1; LSC, laser scanning cytomety; NF-κB, nuclear factor-kappa B; PBS, phosphate buffered saline; PI, propidium iodide; PLP,...

متن کامل

Interaction of Phthalocyanine with Egg albumin and Bovine serum albumin

The interaction of bovine serum albumin ( BSA) and egg albumin with water solublephthalocyanine, cobalt (ΙΙ) 4, 4′ , 4′′, 4′′′- tetrasulfophthalocyanine ( CoTSPc) , has been studiedby the UV- Vis method at pH 7.0 and five different temperatures 20, 25, 30, 35 and 40°C. Theformation constants have been elucidated by using spectrophotometric titration and computerSQUAD program data refinement. Th...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Journal of Experimental Medicine

دوره 89  شماره 

صفحات  -

تاریخ انتشار 1949